Veterinary Chemical · Generic
Reagent kit Calcium
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A veterinary calcium reagent kit is an in vitro diagnostic (IVD) tool used to quantitatively measure the concentration of calcium in animal biological samples. It is vital for diagnosing metabolic conditions like milk fever (hypocalcemia), hyperparathyroidism, and renal diseases.
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Specs & features.
Veterinary calcium assays function by combining a biological sample (typically serum or heparinized plasma) with a specialized chromogenic reagent. Calcium ions in the sample react with a specific dye to form a colored complex. The intensity of this color is measured via spectrophotometry or a microplate reader at a specific wavelength, and it is directly proportional to the total calcium concentration in the animal's blood.
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- Methods of Detection: The most common colorimetric methods utilize dyes such as Arsenazo III (often considered superior for its tolerance to acidic conditions and lack of magnesium interference) or o-Cresolphthalein Complexone (CPC).
- Sample Versatility: They are optimized for use across a wide variety of mammals (dogs, cats, livestock) and can typically process serum, plasma, and in some cases, urine or tissue lysates.
- High Sensitivity & Linearity: These kits offer high analytical sensitivity (often detecting calcium as low as 0.07 mmol/L) and are highly specific to calcium while masking common physiological interferences from lipids (lipemia) or hemoglobin (hemolysis).
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