Veterinary Chemical · Generic
RBC diluting fluid
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RBC diluting fluid is an isotonic laboratory reagent used to dilute whole blood specimens before conducting a manual red blood cell count on a hemocytometer. It ensures the blood is thinned for accurate microscopic viewing while preserving cellular shape and preventing hemolysis (cell bursting).
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Specs & features.
RBC diluting fluid is isotonic with blood; hence hemolysis does not take place. Normal Saline also can be used. But it causes slight creation of red blood cells and allows rouleaux formation.
No specifications have been listed.
- Isotonicity: The fluid matches the osmotic pressure of blood plasma. This ensures that red blood cells neither swell, shrink, nor burst during the mixing and settling process.
- Cell Preservation: It stops the spontaneous breakdown of erythrocytes, yielding a stable cell suspension for accurate microscopic analysis.
- Standardized Dilution: Blood is typically drawn to the 0.5 mark on a Thoma pipette and filled to the 101 mark, achieving a standard 1:200 dilution ratio.
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